Table 2:

Polymerase chain reaction primers used to amplify NR2E1 sequences

	Forward*		Reverse†
Assay	Name	Sequence	Name	Sequence
CE11A	oEMS1988	5′-TACGCCTTAAATCCGAGGTC-3′	oEMS1989	5′-CGATCAAGCATGGTGTCAAG-3′
CE12A	oEMS1990	5′-TGACACCGAGTCTGGAGAAA-3′	oEMS2031	5′-GTCGCCTCCATTATCTGCAC-3′
CE13A	oEMS1994	5′-CAGCTCTGCTTGGGGGAAG-3′	oEMS1995	5′-AAAACGCTTTTCCCCCTCT-3′
CE14A	oEMS1998	5′-TCCTTCTTGCCGTGAAATATAC-3′	oEMS2032	5′-GGAAAACTAGATTGCTGGGAAAT-3′
5′-UTRa	oEMS2033	5′-CCAGGGACGCCCTATTCC-3′	oEMS2034	5′-GAGGAAGAAGGAAGAACAGCA-3′
5′-UTRb	oEMS2035	5′-CCCACACTCTGCATGCCTAT-3′	oEMS2036	5′-GACAGGTGGGTGTCAGTCG-3′
Exon1	oEMS2037	5′-TGTGTCCATATCAAGCAGCA-3′	oEMS2038	5′-CTCCACGAAATGCTCCAACT-3′
CE17B	oEMS2011	5′-GGAGAGCAGAGCGATGTCAC-3′	oEMS2012	5′-TCACGAGACAAGCTGGTTGA-3′
CE19B	oEMS2013	5′-CCTCCCACAGCACAATCTC-3′	oEMS2016	5′-GTCCCAGACTCGTCTCAGGT-3′
Exon2	oEMS1966	5′-TTCGGTGCTAATCCCTTCAG-3′	oEMS1967	5′-AGAGGAAGGGAGAGGTCAGG-3′
Exon3	oEMS1968	5′-GGACTGGCCCTCTTGAAGTA-3′	oEMS1969	5′-TCCCAGCATCTGGAAAGAAG-3′
Exon4	oEMS1970	5′-CTCCCTCAGATTCCCTCTCC-3′	oEMS2039	5′-AACTGGGTGCGTCCCTCT-3′
Exon5	oEMS1972	5′-TACCCACCAATGTCAACTGC-3′	oEMS1973	5′-AACCCACAGGAAGAAGCAAG-3′
Exon6	oEMS1974	5′-TGGGAAAATAAGGGAAAGCTAGA-3′	oEMS1975	5′-ATTTAAATAACAATGCAAGCAGTCA-3′
Exon7	oEMS1976	5′-CTTTCATACAATATAGCCGGTTTACA-3′	oEMS1977	5′-AACATGCAGGTTCCCATAGC-3′
Exon8	oEMS1978	5′-GATTACAGACACATGCCACCAT-3′	oEMS1979	5′-CACCCACCCTGAGAGATAGG-3′
Exon9	oEMS2040	5′-TTCAAGTGTAAGACGTTAGTTTCCA-3′	oEMS2041	5′-CTGTGGCAACCCCCAGTT-3′
3′-UTRa	oEMS2042	5′-AAAGCATTCCAGTAGCTATGACC-3′	oEMS2043	5′-GTTGCCTGGCCTATGGTATT-3′
3′-UTRb	oEMS2044	5′-CATTATTAAGTGGCCTTCAGAACT-3′	oEMS2045	5′-CAGTTTTCGGAAAGGCATTG-3′
3′-UTRc	oEMS2046	5′-CCAGACAGGAAACGAATATGG-3′	oEMS2047	5′-CCTTGTTTCTGGTGGGTGAG-3′

^*5′-TGTAAAACGACGGCCAGT-3′ sequence (-21M13F) was added to the 5′ end of each forward primer to facilitate sequencing.

^†5′-CAGGAAACAGCTATGAC-3′ sequence (M13R) was added to the 5′ end of each reverse primer to facilitate sequencing.