Skip to main content
. 2007 Oct;9(10):812–819. doi: 10.1593/neo.07403

Figure 6.

Figure 6

AE1 knockdown induced cell death and growth inhibition in AGS and SW1116 cells. (A) AGS and SW1116 cells were stably transfected with P2-pSilencer or scrambled-pSilencer 3.1-H1 neo constructs, respectively. After selection by G418, 12 single clones of P2 vector or scrambled vector transfectants—numbered 3, 22, and 36 (AGS; scrambled vector transfectants); 7, 8, and 47 (AGS; P2 vector transfectants); 2, 5, and 7 (SW1116; scrambled vector transfectants); and 1, 4, and 6 (SW1116; P2 vector transfectants)—were isolated and expanded in 96-well plates. Viable cells were counted after 17 days. One thousand cells were then isolated from the same clones and expanded for another 8 days (total, 25 days), and the cells were counted. *Number of cells, < 104. **Number of cells, 0. (B) Another six clones of P2 vector or scrambled vector transfectants of AGS and SW1116 were isolated and expanded. The cell deaths of the P2 vector AGS (a) and SW1116 (c) transfectants or scrambled vector AGS (b) and SW1116 (d) transfectants were observed. The morphologic features of the P2 vector AGS (e) and SW1116 (g) transfectants or the scrambled vector AGS (f) and SW1116 (h) transfectants were examined under a microscope following Wright's staining of the cells.