Table 3.
Selected Genes Differentially Expressed between the GSTIMUL Group and the GINHIB Group.
| P | Regulation in OV-90 Cells with Ascites That Inhibited the Invasion | Probe Set HG-U133a | UniGene | Description | Symbol | Cytoband | GO Biological Process Description | GO Molecular Function Description |
| .011 | Down | 204602_at | Hs.40499 | Dickkopf homolog 1 (Xenopus laevis) | DKK1 | 10q11.2 | Development | Signal transducer activity |
| Wnt receptor signaling pathway | Protein binding | |||||||
| Growth factor activity | ||||||||
| Low-density lipoprotein receptor binding | ||||||||
| .027/.082* | Down | 204337_at | Hs.386726 | Regulator of G-protein signaling 4 | RGS4 | 1q23.3 | Inactivation of MAPK activity | Signal transducer activity |
| 204339_s_at | G-protein-coupled receptor protein signaling pathway | GTPase activator activity | ||||||
| Calmodulin binding | ||||||||
| .044 | Down | 203882_at | Hs.1706 | Interferon-stimulated transcription factor 3, gamma 48 kDa | ISGF3G | 14q11.2 | Transcription | Transcription factor activity |
| Immune response | Protein binding | |||||||
| Cell surface receptor-linked signal transduction | Metal ion binding | |||||||
| Response to virus | ||||||||
| .052 | Down | 202241_at | Hs.444947 | Tribbles homolog 1 (Drosophila) | TRIB1 | 8q24.13 | Protein amino acid phosphorylation | Protein serine/threonine kinase activity |
| Cell proliferation | ||||||||
| Regulation of MAPK activity | ATP binding | |||||||
| .057 | Down | 201041_s_at | Hs.171695 | MAP kinase phosphatase 1 | MKP1 | 5q34 | Protein amino acid | Non-membrane-spanning protein |
| Dephosphorylation | Tyrosine phosphatase activity | |||||||
| Response to oxidative stress | Protein binding | |||||||
| Cell cycle | Hydrolase activity | |||||||
| MAP kinase phosphatase activity | ||||||||
| Protein tyrosine/serine/threonine phosphatase activity | ||||||||
| .066 | Down | 204748_at | Hs.196384 | Cyclooxygenase 2 | COX2 | 1q25.2 | Prostaglandin biosynthesis | Peroxidase activity |
| q25.3 | Cell motility | Prostaglandin-endoperoxide synthase activity | ||||||
| Physiological process | Metal ion binding | |||||||
| Blood pressure regulation | Oxidoreductase activity | |||||||
| Cyclooxygenase pathway | ||||||||
| Keratinocyte differentiation | ||||||||
| Anagen | ||||||||
| Lipid biosynthesis | ||||||||
| Inflammatory response | ||||||||
| .082 | Down | 218853_s_at | Hs.590789 | Motile sperm domain containing 1 | MOSPD1 | Xq26.3 | Structural molecule activity | |
| .011 | Up | 216971_s_at | Hs.434248 | Plectin 1, intermediate filament binding protein 500 kDa | PLEC1 | 8q24 | Cytoskeletal anchoring | Actin binding |
| Structural molecule activity | ||||||||
| .014 | Up | 213002_at | Hs.519909 | Myristoylated alanine-rich protein kinase C substrate | MARCKS | 6q22.2 | Cell motility | Calmodulin binding |
| Actin binding |
The GSTIMUL group consists of OV-90 samples with no FBS, with 5% FBS, or with 5% of the four ascites that stimulated cell invasion (A1592, A1946, A2085, and A2090). The GINHIB group consists of OV-90 samples with 5% of the six ascites that inhibited cell invasion (A1317, A1318, A1322, A1322(2), A1337, and A1835).
*
Corresponding to two different probeset on the Affymetrix HG-U133A GeneChip array.