Figure 1.

Antineoplastic effect of α-adrenoceptor antagonists on several human prostate cancer cell lines. The compound, at the indicated concentration, was added to cells for 48 hours. Then the cells were fixed and stained with SRB. After a series of washings, bound SRB was subsequently solubilized and absorbance was read at a wavelength of 515 nm. Data are expressed as the mean ± SEM of four independent determinations (each in triplicate) (A). IC₅₀ values were calculated as described in the Materials and Methods section (B). PC-3 cells were treated with or without prazosin (30 µM) for the indicated times. Cell morphology was detected by microscopic examination (C). PC-3 cells were treated with or without prazosin (30 µM) for 36 hours. Apoptosis was detected by TUNEL and Hoechst 33342 reaction techniques (D). Scale bar, 20 µm. *IC50 > 100 µM.