Figure 3.

Effect of prazosin on the expression of several cell-cycle regulators. (A and B) PC-3 cells were incubated in the absence or in the presence of prazosin (30 µM) for the indicated times. Then the cells were harvested and lysed for the detection of protein expression with the antibody by Western blot analysis. For Western blot analysis, the amount of proteins (40 µg) was separated by electrophoresis in a 10% or a 15% polyacrylamide gel, transferred to a nitrocellulose membrane, and immunoreacted with the indicated antibody.