Table 2.

PCR primer sequences specific to the target genes and annealing temperatures

Primer	Sequence	Annealing temperatures (°C)	Size (bp)	Cycle
AE2	Sense: 5′-GAAGATTCCTGAGAATGCCT-3′	55.5	181	30
	Antisense: 5′-GTCCATGTTGGCAGTAGTCG-3′
bAE3	Sense: 5′-ATCTGAGGCAGAACCTGTGG-3′	60	418	28
	Antisense: 5′-TTTCACTAAGTGTCGCCGC-3′
AE4	Sense: 5′-AGCGCTTGGACTGCCTTGGTATGT-3′	57	431	30
	Antisense: 5′-AGGGGGAAGATGATGGCTGCAGGGGTAGAC-3′
SLC26A3	Sense: 5′-TGCCACAGCCAACAGAAAAATCAAA-3′	58	330	30
	Antisense: 5′-GGGGGAATGTCGACCAGCAGAG-3′
SLC26A4	Sense: 5′-GTTTACTAGCTGGCCTTATATTTGGACTGT-3′	55	484	30
	Antisense: 5′-AGGCTATGGATTGGCACTTTGGGAACG-3′
SLC26A6	Sense: 5′-TAGGGGAGGTTGGGCCAGGGATGC-3′	60	456	28
	Antisense: 5′-TGCCGGGAAGTGCCAAACAGGAAGAAGTAGAT-3′
SLC26A7	Sense: 5′-CACTGTGTCTGGGATAATGTTGG-3′	65	353	30
	Antisense: 5′-CCAGTTGCAGCACAAACATG-3′
SLC26A8	Sense: 5′-CCAAGACCCAGACCGAGATG-3′	58	150	30
	Antisense: 5′-GAGTCTGAGACTGGGTGGAAGC-3′
SLC26A9	Sense: 5′-TCCAGGTCTTCAACAATGCCAC-3′	58	400	30
	Antisense: 5′-CGAGTCTTGTGCATGTAGCGAG-3′
SLC26A11	Sense: 5′-ATC CCG CCC TTC TCA GTG AC-3′	65	329	28
	Antisense: 5′-TAGTCCAGAGACAGCAGCACCAG-3′
MUC5AC	Sense: 5′-TCCGGCTCCATCTTCTCC-3′	60	680	33
	Antisense: 5′-ACTTGGGCACTGGTGCTG-3′
cornifin-α	Sense: 5′-CATTCTGTCTCCCCCAAAAA-3′	60	172	30
	Antisense: 5′-ATGGGGGTATAAGGGAGCTG-3′
β2 microglobulin	Sense: 5′-CTCGCGCTACTCTCTCTTTCTGG-3′	55	266	23
	Antisense: 5′-GCTTACATCTCTCCATCCCACTTAA-3′