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. Author manuscript; available in PMC: 2008 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2007 Jul 12;1774(9):1148–1155. doi: 10.1016/j.bbapap.2007.06.014

Fig. 4.

Fig. 4

SPR measurements on the influence of Mg2+ concentration on ICAM-1 binding by the αL I domain constructs. Binding was measured for the wild-type (A,B), the L161C/F299C (C,D), E284C/E301C (E,F), and F292A-mutated (G,H) constructs. For comparison the sensorgrams are shown for injection of the αL wild-type, L161C/F299C, and E284C/E301C constructs in the presence of Mg2+ at concentrations between 0.1 mM to 10 mM with the maximal applied I domain concentration at 40 μM for the wild-type construct (panel A), at 4.6 μM for the L161C/F299C construct (panel C), at 2.4 μM for the E284C/E301C construct (panel E), and at 15 μM for the F292A-mutated construct (panel G). The ends of the injection phases are indicated with arrows. In panels B, D, F, H representative equilibrium responses (Req) are shown for each construct (wild-type, L161C/F299C, E284C/E301C, and F292A, respectively) at all tested I domain and Mg2+ concentrations. Solid lines indicate the calculated Req based on the estimated KMg and KMg•I according to Equation (9).