Figure 7. Localization of TRIM5α protein variants.
HeLa cells stably expressing the indicated HA-tagged TRIM5α proteins (or the empty LPCX vector) were fixed and permeabilized. Cells were stained with an anti-HA antibody followed by a secondary anti-rat IgG antibody conjugated with FITC. Stained cells were examined using a confocal fluorescent microscope. The unmodified TRIM5α proteins (wt) are shown in the left column, and the Cluster I-A and Cluster II-A variants of each protein in the middle and right columns, respectively.