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. Author manuscript; available in PMC: 2007 Oct 23.
Published in final edited form as: Circ Res. 2007 Feb 22;100(6):884–893. doi: 10.1161/01.RES.0000260802.75766.00

Figure 1.

Figure 1

Characterization of CD11b-DTR mice. A, Representative flow-cytometric profiles from DTR-FV/B mice treated with 2 doses of 10 ng/g or 15 ng/g DT at 48-hour intervals. Blood was isolated 24 hours later, labeled with F4/80–fluorescein isothiocyanate (FITC) and CD3-R-phycoerythrin (CD3-RPE) antibodies and analyzed by flow cytometry. Absolute F4/80 counts are also shown for each dose of DT as mean±SEM. *P<0.05 (n=3) compared with controls (0 ng/g DT). B, Flow cytometry for Annexin V demonstrating increased circulating Annexin V–positive cells after DT treatment. C and D, Peritoneal macrophages isolated from these mice demonstrate apoptosis on acridine orange staining (orange cells with condensed chromatin) (C) and DNA laddering (D).