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. Author manuscript; available in PMC: 2007 Oct 23.
Published in final edited form as: Circ Res. 2007 Feb 22;100(6):884–893. doi: 10.1161/01.RES.0000260802.75766.00

Figure 2.

Figure 2

DT treatment reduces monocytes and macrophages in transplanted mice. A, ApoE−/− mice were transplanted with CD11b-DTR-EGFP/apoE−/− bone marrow at 8 weeks of age and treated with saline (control) or 10 ng/g DT 3 times per week for 5 weeks. Monocytes were separated by forward scatter/side scatter, and GFP expression was examined by flow cytometry. Nontransplanted apoE−/− mice (right) were also used as a negative control for GFP. B, PCR for DTR in blood of recipient apoE−/− mice before transplant, DTR+/+/apoE−/− donor mice and apoE−/− mice transplanted with DTR+/+/apoE−/− marrow and treated with saline control (BMT control) or DT (BMT DT) from 10 to 22 weeks or 20 to 32 weeks. C, Immunocytochemistry using anti-GFP or control antibody of peritoneal macrophages of mice transplanted with CD11b-DTR/apoE−/− marrow or control apoE−/− mice.