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. Author manuscript; available in PMC: 2007 Oct 23.
Published in final edited form as: J Neurochem. 2005 Jul;94(2):452–458. doi: 10.1111/j.1471-4159.2005.03213.x

Fig 4. SV endocytosis in immature nerve terminals is Ca2+-dependent.

Fig 4

(A) Glutamate release from 2 week old animals was evoked by either 1.3 mM CaCl2 or 1.3 mM BaCl2. Ba2+-dependent glutamate release is displayed (BaCl2 minus CaCl2, n=3 ± SEM). Bar denotes period of BaCl2 addition. (B) FM2-10 was loaded into 2 week old synaptosomes using an S1 stimulation of either 1.3 mM CaCl2 or 1.3 mM BaCl2. The amount of accumulated FM2-10 was quantified with an unloading stimulus of 30 mM KCl (bar). Graph displays Ba2+-dependent FM2-10 loading (BaCl2 minus CaCl2, n=3 ± SEM).