Table 2.
Effects of a single dose of Ampligen administered at various times in relation to virus exposure on survival and brain virus titers in BALB/c mice inoculated with Banzi virus or Semliki Forest virus. All mice received an approximately LD90 dose of virus inoculum.
| Uninfected toxicity control | Infected, treated | |||||
|---|---|---|---|---|---|---|
| Compound
Virus |
Virus | Dose | Surv/Total | Surv/Total | MDDa | Brain Virus
Titersbc |
| Ampligen (24 h pre) | Banzi | 1 mg/kg | 3/3 | 9/10*** | 14.0 | 2.3 ± 0.1*** |
| Ampligen (4-6 h pre) | Banzi | 1 mg/kg | 3/3 | 7/10*** | 12.3 ± 1.5 | 3.8 ± 1.0*** |
| Ampligen (24 h post) | Banzi | 1 mg/kg | 3/3 | 0/10 | 12.8 ± 3.1* | 7.0 ± 2.1 |
| Placebo (24 h pre) | Banzi | - | 3/3 | 0/20 | 10.4 ± 1.8 | 9.1 ± 0.1 |
|
| ||||||
| Normal Control | - | - | 3/3 | - | - | - |
|
| ||||||
| Ampligen (24 h pre) | Semliki Forest | 1 mg/kg | 3/3 | 4/10 | 9.0 ± 1.1* | 6.2 ± 1.9 |
| Ampligen (4-6 h pre) | Semliki Forest | 1 mg/kg | 3/3 | 7/10*** | 7.7 ± 1.5 | 5.5 ± 1.5+ |
| Ampligen (24 h post) | Semliki Forest | 1 mg/kg | 3/3 | 3/10 | 7.4 ± 1.8 | 7.9 ± 1.1 |
| Placebo (24 h pre) | Semliki Forest | - | 3/3 | 2/20 | 7.6 ± 1.3 | 7.4 ± 1.2 |
|
| ||||||
| Normal Control | - | - | 3/3 | - | - | - |
a
Mean day to death of mice dying before day 21
b
Log10 infectious doses/g of tissue.
c
Banzi virus inoculated animals assayed on 8 dpi. Semliki Forest virus inoculated animals assayed on 6 dpi.
*
p<0.05,
***
p<0.001 compared to placebo treated controls,
+
p<0.05 compared to Ampligen treatment 24 h after virus inoculation