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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Exp Cell Res. 2007 Apr 19;313(13):2920–2936. doi: 10.1016/j.yexcr.2007.03.043

Fig. 2. Endogenous and ectopic hPARG expression in HeLa cells.

Fig. 2

(A) Immunoblot analyses of pEhP111/pEhP102 transfected HeLa cells using two anti-peptide antibodies generated in rabbits that are either N-terminus-specific (ab9882) or C-terminus-specific (ab9877). Both antibodies recognize the overexpressed PARG protein but, as expected, only ab9877 and not ab9882 specifically detects endogenous PARG protein in the range of 55-60 kDa. (B) PCR amplification of PARG splice variants obtained from reversely transcribed HeLa cell total RNA. Using the same primer sets as in Fig. 1, the bands representing hPARG111 (1), hPARG102 /hPARG99 (2), and the novel hPARG60 (3) were reproducibly amplified.