Figure 1.

Effects of SC19220 and AH6809 on the progesterone responses to PGE₂ by human granulosa–lutein cells. Progesterone concentrations in medium collected after a 24-h incubation in the presence of 0–3000 nM PGE₂, either in the absence (circle) or in the presence of 10 μM SC19220 (square) or 10 μM AH6809 (triangle). Values are the mean±s.e.m. for three independent experiments with quadruplicate determinations in each experiment. *P<0·05, **P<0·01, ***P<0·001 relative to 0 nM PGE₂ in the absence or presence of the PTGER receptor antagonist, as appropriate. ^aP<0·05, ^cP<0·001 relative to cells treated with the same PGE₂ concentration in the absence of PTGER receptor antagonists (one-way ANOVA plus Bonferroni's post hoc multiple comparison test).