Table 1.

Induction of phosphoproteome by control (C), leptin (L), TNFα (T), or co-treatment (TL). In this list of identified phophoproteins and their in-gel densities, the relative spot intensity, which reflects the level of expression, is normalized to volume, and expressed as PPM of the total spot volume within the gel. NCBI, %, pI, and MW denote NCBI accession number, sequence coverage, isoelectric point (theoretical), and molecular weight in kD (theoretical) for each protein. In the gel, proteins were usually found within ±0.5 pI units and ± 5 kD range from their theoretical values. Sp. denotes taxon: b - bovine, h - human, m - mouse, p - pig, r - rat.

No.	Sp.	Protein	NCBI	%	pI	MW	C	T	L	TL
1	m	brain α-tropomyosin (TMBr-3)	13270471	37	4.7	32.72	1671	2292	412	1182
2	p	chain b, tubulin α-β dimer	3745822	22	5.2	48.33	614	672	2449	1226
3	m	syntaxin 7	7949144	19	5.6	29.97	679	1038	2027	2036
4	m	villin 2	2832989	23	5.9	69.53	341	1928	2147	531
5	b	proteasome subunit, α type 1	21465647	44	6.1	29.82	2447	2884	2173	10
6	m	proteasome subunit, β type 2	6755200	55	6.5	23.06	4402	4664	3037	1020
7	m	proteasome subunit Lmp7	673450	56	6.9	23.26	1109	1242	1193	10
8	m	proteasome subunit, α type 2	6679497	59	8.6	26.02	4216	7358	4443	1262
9	r	proteasome subunit, α type 3	8394066	48	5.3	28.63	4677	100	1737	906
10	h	proteasome 26S subunit, type 2	4506209	40	5.7	49.02	351	1991	1242	335
11	m	translation elongation factor 1δ-b	12963597	42	4.9	31.39	767	2364	783	1086
12	m	elongation factor Tu, mitochondrial	20843609	34	7.3	49.89	739	1234	2373	2081
13	m	translation elongation factor 1β	12856949	30	4.9	28.82	244	1173	2544	1596
14	m	hnRP C	13435678	37	5.0	32.26	453	100	100	567
15	m	hnRP A2/B1	21071091	47	8.7	36.02	10	446	588	10
16	m	poly(rC) binding protein 1	6754994	42	6.7	37.99	849	100	560	785
17	h	septin 7	4502695	25	9.0	49.05	10	10	725	10
18	h	JM2 protein	14759872	25	9.2	44.24	10	10	498	10
19	m	aldehyde dehyrdogenase 8, A1	23512292	22	7.8	54.39	10	10	100	10
20	r	serum-inducible kinase	13929172	16	9.0	78.99	10	638	676	1117
21	r	Cytochrome P450, subfamily IIC	9506529	25	7.8	57.59	1264	3946	10	2970
22	h	ketohexokinase, isoform a	4557693	26	5.6	33.11	630	420	10	442
23	r	collapsin response mediator 2	1351260	40	6.0	62.66	1806	3974	2226	813
24	h	follistatin precursor	120547	18	5.9	39.52	521	2586	2418	576
25	m	similar to septin 6	20836152	21	5.9	52.86	554	2507	1903	827
	m	TNFA-IP3 interacting protein 2	20532344	21	6.0	49.82
	m	pyruvate dehydrogenase kinase	19526816	21	6.1	46.28
	m	PI3-kinase p85-gamma subunit	9910789	16	5.7	54.74
26	m	transaldolase 1	20846995	46	6.6	37.54	150	1121	1050	150
27	m	transaldolase 1	20846995	33	6.6	37.54	3872	6217	6231	7766
28	r	transaldolase 1	13929152	38	6.6	37.63	791	3634	2192	4711
29	m	transaldolase 1	20846995	38	6.6	37.54	749	3777	532	5595
30	m	kinesin family member C1 (NS)	16195832	21	6.5	47.06	150	1795	740	1073
31	h	helicase-like protein 2	13514813	22	6.7	73.65	261	1819	1129	394