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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Neuropharmacology. 2007 Apr 29;53(1):92–100. doi: 10.1016/j.neuropharm.2007.04.005

Figure 2. NMDA/low Mg2+-treatment increases S-nitrosylation of NSF.

Figure 2

A, NSF in lysates from NMDA/low Mg2+-treated and untreated hippocampal slices, was assayed for levels of S-nitrosylation. After performing a biotin-switch protocol, S-nitrosylated proteins were isolated and probed for NSF by Western blot. While NSF levels are shown to be equal in total protein fractions, the S-nitrosylated lanes show that NMDA/low Mg2+ increased the amount of modified NSF. B, Quantitation of A. The unnormalized data is graphed in arbitrary units (AU). (n= 3; * p< 0.05)