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. Author manuscript; available in PMC: 2008 Oct 19.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Aug 7;362(2):354–359. doi: 10.1016/j.bbrc.2007.07.154

Figure 2. Activation of AMPK by AICAR is not associated with an increase in p38 MAPK phosphorylation in skeletal muscle.

Figure 2

Rat EDL muscles were isolated and preincubated in KRB buffer for 20 min. The muscles were then incubated with 2 mM AICAR for 40 min. Phosphorylation of AMPK (A) and p38 MAPK (B) were determined by immunoblotting using phospho-specific antibodies. Data are the means ± SEM. n = 4/group. (C); Mice were injected intraperitoneal with AICAR (1mg/g). Gastrocnemius muscles were harvested at each time point indicated. Phosphorylation of AMPK and p38 MAPK were determined by immunoblotting using phosphospecific antibodies. Representative images are shown.