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. Author manuscript; available in PMC: 2008 Oct 19.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Aug 7;362(2):354–359. doi: 10.1016/j.bbrc.2007.07.154

Figure 4. Muscle contraction-induced p38 MAPK phosphorylation in AMPK activity in α2i TG mice.

Figure 4

Wild type and α2i TG mice were anesthetized, and sciatic nerves were exposed. One leg was electrically stimulated for 10 min to induce muscle contractions, and the other leg served as sham control. Gastrocnemius muscles were dissected. In vitro kinase assay was done to determine AMPKα1 (A) and α2 (B) activities after immunoprecipitation with their specific antibodies as described in Material and Methods. The same protein samples used for the kinase assays were used for immunoblotting of phospho-p38 MAPK (C) and phospho-ATF2 (D). Data are the means ± SEM. n = 5-9/group.