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. 2007 Oct 25;117(11):3306–3315. doi: 10.1172/JCI31763

Figure 6. Inhibition of TGF-β signaling by IN-1130 does not significantly affect the distribution of CD4 T cell subsets.

Figure 6

C57BL/6 mice were immunized with MOG35–55 emulsified in CFA plus PT and treated with IN-1130 or PBS (i.p.) daily from 1–14 dpi. T cells were isolated from spinal cords and spleens of 6 mice combined at 20 dpi. Cells were stained with antibodies against CD4 in combination with IL-4, IFN-γ, Foxp3, and IL-17 and analyzed by flow cytometry. (A) Dot plots of IL-17 expression in CD4+ T cells from spinal cord (top panels) or spleen (bottom panels). (B and C) Percentages of CD4 T cell subsets expressing the indicated intracellular markers from spinal cord (B) and spleen (C). No significant differences were observed in any of the percentages between IN-1130 (black bars) and PBS-treated (white bars) mice by FlowJo population comparison; T(X) = 0.