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. Author manuscript; available in PMC: 2007 Oct 23.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Feb 15;851(1-2):186–210. doi: 10.1016/j.jchromb.2007.02.011

Fig. 4.

Fig. 4

An example of HPCE analysis of NO metabolites in isolated neurons, putative NO-ergic B2, peptidergic B4, and LPeD1 cells examples are shown. (A) CZE-LIF separation and detection of arginine/citrulline ratio in the samples of isolated B2 and B4 neurons. Arginine and citrulline peaks are identifiable at electropherograms, but citrulline levels are very low in B4 neuron, see magnified superimposed traces in insertion. Sample was derivitised with fluorescamine and separated in 40 cm beyond the detector point long capillary using a borate/SDS BGE. LIF setup included 350–356 nm Ar/Kr ion laser microscope objective spatial filter and photomultiplier tube. (B) Electrophoretic separation/detection of NO2−/NO3− in selected identifiable molluscan neurons. CZE-contact CD was performed in arginine/borate BGE (pH 9.5) with addition of 0.5 mM TTAOH electro-osmotic modifier. Samples were clean up with SPME techniques and introduced using ITP staking injection. The separation was carried at −28 kV. Reprinted from [101], Journal of Inorganic Biochemistry 99, L.L. Moroz, R.L. Dahlgren, D. Boudko, J.V. Sweedler, P. Lovell, Direct single cell determination of nitric oxide synthase related metabolites in identified nitrergic neurons, 929–939, 2005, with permission from Elsevier.