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. Author manuscript; available in PMC: 2007 Oct 23.
Published in final edited form as: J Biol Chem. 2006 Nov 29;282(5):2891–2898. doi: 10.1074/jbc.M608057200

FIGURE 1. Ephx2-null mice of the NIH and BI colonies lack a complete sEH gene and express no detectable sEH protein.

FIGURE 1

A, representative genotype analysis of wild type and Ephx2-null mice from the NIH colony. Negative and positive controls show no band product or a 290-bp band, respectively. Genomic DNA of sEH amplified from mouse tail shows a 290-bp gene product of sEH from NIH colony wild type. The Ephx2-null mice show a higher gene product of 560 bp from the neomycin resistant gene. Genotypic analysis was performed in all mice in each breeding colony. B, tissue distribution of sEH protein from wild type and Ephx2-null mice from both colonies. Immunoblots demonstrate expression of the 62.5 kDa protein in heart (H), kidney (K), and liver (L) of wild type (+/+) and lack of sEH protein expression in Ephx2-null mice (−/−) of both colonies.