TABLE 1. sEH specific activities in liver, kidney, and heart tissue homogenates from the wild-type and Ephx2-null mice.

Tissues were extracted as described under “Experimental Procedures” and examined for sEH activity in S9 fractions using tDPPO as a substrate. Ephx2-null mice show no epoxide hydrolase activity toward tDPPO in any of examined tissues in two different Ephx2-null mouse colonies originated from NIH or BI. All results are means ± S.D. of three separate experiments as described under “Experimental Procedures.”

Genotype	BI colony		NIH colony
	Ephx2+/+	Ephx2−/−	Ephx2+/+	Ephx2−/−
	nmol diol produced × min−1 × mg protein−1		nmol diol produced × min−1 × mg protein−1
Liver	32 ± 2	<0.1	38 ± 5	<0.01
Kidney	18 ± 3	<0.1	16 ± 3	<0.01
Heart	14 ± 2	<0.1	1.4 ± 0.2	<0.04