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. Author manuscript; available in PMC: 2008 Jun 1.
Published in final edited form as: Arch Biochem Biophys. 2007 Apr 25;462(1):62–73. doi: 10.1016/j.abb.2007.04.010

Fig. 4.

Fig. 4

Depletion of derlin 1 induces accumulation of α1-antitrypsin but not of αTCR. (A) RNAi of derlin 1 with two different siRNAs (der1-1 and der1-2) induces a decrease of the levels of glycosylated αTCR (arrow) while at the same time induces accumulation of glycosylated α1-antitrypsin (arrowhead). RNAi of derlin 2 does not affect the levels of neither αTCR nor α1-antitrypsin, while treatment with MG132 induces accumulation of both substrates, mostly in the deglycosylated form (stars). Equal protein loading is shown by the actin blot. (B) The effectiveness of the knockdown of derlins is demonstrated by semiquantitative RT-PCR, with actin as a control of equal RNA loading.