. Author manuscript; available in PMC: 2008 Oct 1.

Published in final edited form as: Eur J Pharmacol. 2007 Jun 12;571(2-3):129–137. doi: 10.1016/j.ejphar.2007.06.001

Table 2.

Effect of morphine, THC or low-dose combination treatment on E_max and EC₅₀ values of cannabinoid CB₁ receptor-stimulated [³⁵S]GTPγS binding

Region	Vehicle Control		Drug Treated
Spinal Cord	E_max (% Con)	EC₅₀ (nM)	E_max (% Con)	EC₅₀ (nM)
Morphine	100 ± 18	93 ±14	106 ± 9.4	51 ± 20
THC	100 ± 11	40 ± 4.8	50 ± 6.4^a	190 ± 45
Combo	100 ± 21	57 ± 9.7	89 ± 10	50 ± 17
Periaqueductal Gray	E_max (% Con)	EC₅₀ (nM)	E_max (% Con)	EC₅₀ (nM)

Morphine	100 ± 8.7	219 ± 42	103 ± 14	273 ± 34
THC	100 ± 4.8	148 ± 41	73 ± 6.6^a	295 ± 95
Combo	100 ± 16	215 ± 67	91 ± 7.5	157 ± 31

Membranes were incubated with 0.1 nM [³⁵S]GTPγS, 30 μM GDP and varying concentrations of WIN55,1212-2, as described in Methods. Data are E_max and EC₅₀ values derived from the concentration-effect curves shown Fig. 2.

Significantly different from vehicle control by Bonferoni-adjusted Student's t-test.