Table 2.
Genes showing significant differences in expression between rapid-forming PCTs (ABPC and ABLMYCPC) and slow-forming PCTs (TEPC, IL6PC and KiPC)
| Higher in slow-forming PCT (TEPC, IL6PC, KiPC | |||||||
| Gene function | Rapid PCT | Slow PCT | Fold difference (slow/rapid) | Description | Unigene ID | Gene symbol | p-value |
| Angiogenesis | |||||||
| 308.4 | 495.6 | 1.6 | Neogenin | Mm.42249 | Neo1 | 1.05E-05 | |
| Apoptosis | |||||||
| 2872.8 | 5249.1 | 1.8 | RIKEN cDNA A630035D09 gene | Mm.238213 | Bcl2l1 | 4.67E-05 | |
| Cell cycle | |||||||
| 583.6 | 2799.1 | 4.8 | Megakaryocyte-associated tyrosine kinase | Mm.2918 | Matk | < 1E-07 | |
| 270.1 | 912.6 | 3.4 | Protein kinase C, zeta | Mm.28561 | Prkcz | < 1E-07 | |
| 208.1 | 364.3 | 1.8 | Acid phosphatase, prostate | Mm.19941 | Acpp | 2.10E-06 | |
| 280.7 | 473.5 | 1.7 | Cyclin D1 | Mm.273049 | Ccnd1 | 7.12E-05 | |
| 1484.3 | 2441.1 | 1.6 | Casein kinase 1, epsilon | Mm.30199 | Csnk1e | 3.50E-06 | |
| Immune response | |||||||
| 204.1 | 535.4 | 2.6 | Prolactin receptor | Mm.10516 | Prlr | 5.4E-06 | |
| 124 | 267.7 | 2.2 | Transforming growth factor, beta receptor III | Mm.200775 | Tgfbr3 | 1.2E-06 | |
| 2872.8 | 5249.1 | 1.8 | RIKEN cDNA A630035D09 gene | Mm.238213 | Bcl2l1 | 4.67E-05 | |
| 707.2 | 1243.1 | 1.8 | AU RNA binding protein/enoyl-coenzyme A hydratase | Mm.252034 | Auh | 3.2E-06 | |
| 1000.1 | 1751.7 | 1.8 | Aldehyde dehydrogenase 2, mitochondrial | Mm.284446 | Aldh2 | 3.56E-05 | |
| 2990.5 | 5228.6 | 1.7 | Glucosidase, beta, acid | Mm.5031 | Gba | < 1E-07 | |
| 942.5 | 1629.7 | 1.7 | AU RNA binding protein/enoyl-coenzyme A hydratase | Mm.252034 | Auh | < 1E-07 | |
| 7349 | 12562.5 | 1.7 | CCAAT/enhancer binding protein (C/EBP), beta | Mm.347406 | Cebpb | 1E-07 | |
| 752.1 | 1285.1 | 1.7 | Profilin 1 | Mm.2647 | Pfn1 | 3.5E-06 | |
| 912.2 | 1538.3 | 1.7 | Aldehyde dehydrogenase 2, mitochondrial | Mm.284446 | Aldh2 | 6.2E-05 | |
| 2311.2 | 3862.5 | 1.7 | Epidermal growth factor receptor pathway substrate 15 | Mm.318250 | Eps15 | 1.24E-05 | |
| 166.2 | 274.5 | 1.7 | Microphthalmia-associated transcription factor | Mm.333284 | Mitf | 7.9E-06 | |
| 722.3 | 1097.6 | 1.5 | Amyloid beta (A4) precursor protein-binding, family B, member 1 | Mm.38469 | Apbb1 | 7E-06 | |
| 749.6 | 1135 | 1.5 | Glutathione synthetase | Mm.252316 | Gss | 7.61E-05 | |
| Transcription factor | |||||||
| 294 | 803.5 | 2.7 | AT motif binding factor 1 | Mm.196564 | Atbf1 | < 1e-07 | |
| 247.9 | 639.4 | 2.6 | E74-like factor 3 | Mm.3963 | Elf3 | 6.80E-06 | |
| 844.6 | 1883 | 2.2 | Pre B-cell leukemia transcription factor 1 | Mm.43358 | Pbx1 | < 1E-07 | |
| 765.9 | 1554.6 | 2.0 | POU domain, class 2, transcription factor 2 | Mm.208700 | Pou2f2 | 2.30E-06 | |
| 7349 | 12562.5 | 1.7 | CCAAT/enhancer binding protein (C/EBP), beta | Mm.347406 | Cebpb | 1.00E-07 | |
| 162.2 | 252.2 | 1.6 | RIKEN cDNA 2310011G17 gene | Mm.254233 | 2310011G17 Rik | 6.55E-05 | |
| Signal transduction | |||||||
| 583.6 | 2799.1 | 4.8 | Megakaryocyte-associated tyrosine kinase | Mm.2918 | Matk | < 1E-07 | |
| 270.1 | 912.6 | 3.4 | Protein kinase C, zeta | Mm.28561 | Prkcz | < 1E-07 | |
| 1484.3 | 2441.1 | 1.6 | Casein kinase 1, epsilon | Mm.30199 | Csnk1e | 3.50E-06 | |
| 456.5 | 729.4 | 1.6 | Homeodomain interacting protein kinase 3 | Mm.257925 | Hipk3 | 2.11E-05 | |
| Development | |||||||
| 844.6 | 1883 | 2.2 | Pre B-cell leukemia transcription factor 1 | Mm.43358 | Pbx1 | < 1E-07 | |
| Higher in rapid-forming PCT (ABPC and ABLMYCPC) | |||||||
| Gene function | Rapid PCT | Slow PCT | Fold difference (rapid/slow) | Description | Unigene ID | Gene symbol | p-value |
| Angiogenesis | |||||||
| 1868.3 | 902.8 | 2.1 | Plasminogen activator, urokinase | Mm.4183 | Plau | 8.29E-05 | |
| 9821.9 | 4360.2 | 2.3 | CD53 antigen | Mm.316861 | Cd53 | 3.40E-06 | |
| Apoptosis | |||||||
| 2588.3 | 1681.2 | 1.5 | BCL2-antagonist/killer 1 | Mm.2443 | Bak1 | 9.11E-05 | |
| Cell cycle | |||||||
| 2231.6 | 1126.6 | 2.0 | Proliferation-associated 2G4 | Mm.4742 | Pa2g4 | 2.76E-05 | |
| 4858.7 | 2552.3 | 1.9 | Adenylate kinase 2 | Mm.29460 | Ak2 | < 1E-07 | |
| 10477 | 6082.1 | 1.7 | CDC28 protein kinase regulatory subunit 2 | Mm.222228 | Cks2 | < 1E-07 | |
| 1371.5 | 869.3 | 1.6 | Casein kinase II, alpha 2, polypeptide | Mm.51136 | Csnk2a2 | 1.90E-06 | |
| Immune response | |||||||
| 3568.1 | 386 | 9.2 | CD6 antigen | Mm.290897 | Cd6 | 2.00E-07 | |
| 984.7 | 186.1 | 5.3 | Tubulin, alpha 3 | Mm.287784 | Tuba3 | < 1E-07 | |
| 2661.9 | 718.2 | 3.7 | BH3 interacting domain death agonist | Mm.235081 | Bid | < 1E-07 | |
| 7492.6 | 2554.5 | 2.9 | Acetyl-Coenzyme A dehydrogenase, medium chain | Mm.10530 | Acadm | < 1E-07 | |
| 3195.6 | 1346.2 | 2.4 | Inositol (myo)-1(or 4)-monophosphatase 1 | Mm.183042 | Impa1 | < 1E-07 | |
| 2455 | 1064.3 | 2.3 | RAS-related protein-1a | Mm.333868 | Rap1a | < 1E-07 | |
| 9821.9 | 4360.2 | 2.3 | CD53 antigen | Mm.316861 | Cd53 | 3.40E-06 | |
| 7479.7 | 3365.9 | 2.2 | Transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) | Mm.207996 | Tap1 | < 1E-07 | |
| 7347.8 | 3535.9 | 2.1 | Superoxide dismutase 1, soluble | Mm.276325 | Sod1 | < 1E-07 | |
| 365.9 | 179.9 | 2.0 | Growth factor independent 1 | Mm.2078 | Gfi1 | 1.30E-05 | |
| 2231.6 | 1126.6 | 2.0 | Proliferation-associated 2G4 | Mm.4742 | Pa2g4 | 2.76E-05 | |
| 1939.9 | 985.5 | 2.0 | RAS-related protein-1a | Mm.333868 | Rap1a | 4.00E-07 | |
| 450.3 | 261.9 | 1.7 | Ets variant gene 4 (E1A enhancer binding protein, E1AF) | Mm.5025 | Etv4 | 1.40E-06 | |
| 3031.7 | 1830.6 | 1.7 | Wiskott-Aldrich syndrome homolog (human) | Mm.4735 | Was | 5.13E-06 | |
| 3136.7 | 1944.6 | 1.6 | Chromosome segregation 1-like (S. cerevisiae) | Mm.22417 | Cse1l | 2.67E-05 | |
| 5738.7 | 3586.7 | 1.6 | Survival motor neuron 1 | Mm.2025 | Smn1 | 2.75E-05 | |
| 395.2 | 252.8 | 1.6 | Dihydrofolate reductase | Mm.23695 | Dhfr | 2.70E-06 | |
| 2017.5 | 1297.8 | 1.6 | FK506 binding protein 12-rapamycin associated protein 1 | Mm.21158 | Frap1 | 8.46E-05 | |
| 2588.3 | 1681.2 | 1.5 | BCL2-antagonist/killer 1 | Mm.2443 | Bak1 | 9.11E-05 | |
| 4410.1 | 2873.4 | 1.5 | Ribonucleotide reductase M1 | Mm.197486 | Rrm1 | 5.14E-05 | |
| 5971.6 | 3940.2 | 1.5 | Integrin beta 4 binding protein | Mm.271674 | Itgb4bp | 6.94E-05 | |
| 18860.1 | 12494.5 | 1.5 | Triosephosphate isomerase 1 | Mm.4222 | Tpi1 | 8.30E-06 | |
| Transcription factor | |||||||
| 6231.6 | 1885.8 | 3.3 | Lymphoblastomic leukemia | Mm.4925 | Lyl1 | < 1E-07 | |
| 3274.2 | 1544.3 | 2.1 | Chromodomain helicase DNA binding protein 1 | Mm.8137 | Chd1 | < 1E-07 | |
| 450.3 | 261.9 | 1.7 | Ets variant gene 4 (E1A enhancer binding protein, E1AF) | Mm.5025 | Etv4 | 1.40E-06 | |
| 3136.7 | 1944.6 | 1.6 | Chromosome segregation 1-like (S. cerevisiae) | Mm.22417 | Cse1l | 2.67E-05 | |
| Signal transduction | |||||||
| 4858.7 | 2552.3 | 1.9 | Adenylate kinase 2 | Mm.29460 | Ak2 | < 1E-07 | |
| 10477 | 6082.1 | 1.7 | CDC28 protein kinase regulatory subunit 2 | Mm.222228 | Cks2 | < 1E-07 | |
| 3031.7 | 1830.6 | 1.7 | Wiskott-Aldrich syndrome homolog (human) | Mm.4735 | Was | 5.13E-05 | |
| 1371.5 | 869.3 | 1.6 | Casein kinase II, alpha 2, polypeptide | Mm.51136 | Csnk2a2 | 1.90E-06 | |
| DNA replication | |||||||
| 3805.2 | 2420.4 | 1.6 | Small nuclear ribonucleoprotein polypeptide A' | Mm.821 | Snrpa1 | < 1e-07 | |
| 8247.5 | 4325.9 | 1.9 | Replication protein A1 | Mm.180734 | Rpa1 | 4.20E-06 | |
Class comparison between slow-forming PCTs (TEPC, IL6PC and KiPC) and rapid-forming PCTs (ABPC and ABLMYCPC) by two-sample t-test generated 1195 genes showing significant differences (p- value < 0.001). Among these 1195 genes, the genes showing more than 1.5 fold increase and p-value < 0.0001 were selected in this table. The genes in each category of gene function were assigned based on the annotation of CGAP.