Fig. 3.
CoREST146–482 polypeptide displaces HDAC1 from the HDAC1–CoREST–REST complex. HEK 293 cells were transfected with plasmids encoding the truncated CoREST polypeptides tagged with Myc. After 40 h of incubation, the cells were harvested and lysed. The immune precipitates (IPs) obtained with polyclonal anti-HDAC1 antibody (A) or polyclonal CoREST antibody (B) were collected as described in Experimental Procedures, solubilized, electrophoretically separated in a denaturing gel, and reacted with monoclonal antibody against CoREST (A) or anti-HDAC1 or anti-REST antibodies (B).