Fig. 3.

Time-lapse imaging of glycan trafficking using an Alexa Fluor 488 derivative of DIFO. (A–H) CHO cells were incubated with 100 μM Ac₄ManNAz (A–D) or 100 μM Ac₄ManNAc as a negative control (E–H) for 3 days and subsequently labeled with 100 μM DIFO-488 at 37°C for 1 min. (I–N) Time-lapse imaging of a single cell from the previous experiment over 1 h at 25°C (I–M, Ac₄ManNAz; N, Ac₄ManNAc).