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. 2007 Oct 17;104(43):16898–16903. doi: 10.1073/pnas.0702761104

Fig. 1.

Fig. 1.

NO-mediated cell migration in an ex vivo model of wound-driven angiogenic response is MMP-9-dependent. (A) Conditioned media of untreated cells or cells treated with 10 μM Sper/NO were applied to an ex vivo model of wound-driven angiogenesis, demonstrating increased vascular cell migration as indicated by the outgrowth of vascular cells (arrow) away from the perimeter of explanted tissue. (B) Quantitation of vascular cell migration in explanted tissue exposed to conditioned media of control and Sper/NO-treated ANA-1 cells shown in A, as well as deactivated Sper/NO control media and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ)-treated cells with or without Sper/NO. (C) Enhanced vascular cell migration is suppressed in conditioned media from MMP-9 knockdown cells treated with Sper/NO. (D) Knock down of MMP-9 translation by using MMP-9 antisense morpholino completely inhibits MMP-9 translation, as demonstrated by gel zymography. In contrast, the scrambled control oligo does not affect MMP-9 translation. Symbols indicate statistical significance when compared with conditioned media of untreated control: *, P < 0.001; **, P < 0.05.