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. 2007 Oct 17;104(43):16898–16903. doi: 10.1073/pnas.0702761104

Fig. 3.

Fig. 3.

NO suppression of endogenous TIMP-1 inhibitor is sGC-dependent. (A) Exogenous NO suppresses TIMP-1 levels in conditioned media from Sper/NO-treated ANA-1 cells. (B) Dose-dependent TIMP-1 suppression by 8-bromo-cGMP suggests that low-dose NO effects are sGC-dependent. (C) Inhibition of NO and sGC signaling by thrombospondin-1 (TSP-1) abolished NO-mediated TIMP-1 suppression. All results are representative of n = 3 blots. (D–F) Quantitation of results shown in A–C, respectively. (G) Inverse modulation of MMP activity and TIMP-1 protein levels in the conditioned media of Sper/NO-treated cells suggests perturbation of MMP-9/TIMP-1 balance by NO. Symbols indicate statistical significance when compared with untreated control: *, P < 0.05; **, P < 0.01.