Fig. 2.

Ectopic expression of HOXB13 in murine T1 ovarian cancer cells. (A) RT-PCR detection of endogenous and ectopic HOXB13 in T1 cells infected with the control RCAS-GFP retrovirus (T1-GFP) and T1 cells infected with the RCAS-HOXB13 retrovirus (T1-HOXB13). (B and C) Western blotting (B) and immunofluorescence (C) detection of the human HOXB13 protein by using a rabbit polyclonal antibody to HOXB13. (D and E) The morphology of T1-GFP and T1-HOXB13 cells in monolayer culture grown at low (D) and high (E) cell densities. (F) Representative images of duplicate independent assays of cell proliferation in soft agar. (G) Representative images of TUNEL staining in T1-GFP and T1-HOXB13 cells grown in monolayer culture at high cell density. (H and I) Growth curve plot of cells (×10⁴) (H) and cell cycle analysis (I) of T1-GFP and T1-HOXB13 cells; data are representative of duplicate independent experiments.