Fig. 2.

Acetyl-CoA and NADP(H) levels are highly periodic during the YMC. (A) Acetyl-CoA and NADP(H) concentrations over two consecutive metabolic cycles. Note that both acetyl-CoA and total NADP(H) periodically increase in concentration at the end of the RC phase and into the Ox phase. (B) Temporal expression profiles of the three NAD(H) kinases in yeast. Expression data are from ref. 12. (C) Cells lacking glucose-6-phosphate dehydrogenase (Δzwf1, which cannot use the pentose phosphate pathway to synthesize NADPH) do not exhibit metabolic cycles during continuous growth. Shown is the dissolved oxygen trace of a WT and Δzwf1 strain for ≈40 h after the start of continuous mode. x-axis tick marks denote 5-h time intervals.