Fig. 2.
Comparison of expression patterns of key cell cycle-regulated genes in slowly growing [YMC (14), Left] vs. rapidly growing [cdc15, alpha, and cdc28 (1, 5)] yeast cultures. (Upper) G2 cyclin CLB1, mitotic transcription factor SWI5, and G1 cyclin CLN3. (Lower) Mitotic cyclin PCL9 and MCM subunit MCM3. Environment-dependent G1 phase is ≈10 times longer in YMC than found in previous studies (cdc15, alpha, and cdc28 synchronization), allowing the expression of key cell cycle genes to be timed with higher accuracy.
