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. 2007 Oct 11;104(43):17058–17062. doi: 10.1073/pnas.0707958104

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© 2007 by The National Academy of Sciences of the USA

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Fig. 2. — SNO-Hb and bioactivity of banked RBCs. (A) Cohort A: SNO-Hb concentrations (n = 4–10), expressed per Hb tetramer, and corresponding vasodilatory activity of RBCs [expressed as changes in tension in rabbit aortic rings (mean ± SD; n = 11–22; arc-sine transformed)] for fresh (Day 0) and stored blood. Bars marked with are time points where SNO-Hb levels were significantly reduced compared with fresh blood (P < 0.05). Similarly, vasorelaxations marked with were significantly less (P < 0.01) than those produced by fresh RBCs. Each time point is an independent sampling comprising different individuals. (B) Cohort B: Change in SNO-Hb concentration over time from serially sampled blood bags obtained at day 2 (n = 6); SNO-Hb is significantly reduced at all time points compared with fresh venous blood. Blood in cohorts A and B was obtained from the same commercial source.

Inline graphic — SNO-Hb and bioactivity of banked RBCs. (A) Cohort A: SNO-Hb concentrations (n = 4–10), expressed per Hb tetramer, and corresponding vasodilatory activity of RBCs [expressed as changes in tension in rabbit aortic rings (mean ± SD; n = 11–22; arc-sine transformed)] for fresh (Day 0) and stored blood. Bars marked with are time points where SNO-Hb levels were significantly reduced compared with fresh blood (P < 0.05). Similarly, vasorelaxations marked with were significantly less (P < 0.01) than those produced by fresh RBCs. Each time point is an independent sampling comprising different individuals. (B) Cohort B: Change in SNO-Hb concentration over time from serially sampled blood bags obtained at day 2 (n = 6); SNO-Hb is significantly reduced at all time points compared with fresh venous blood. Blood in cohorts A and B was obtained from the same commercial source.