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Plasma BNP and LV gene expression. (A–C) Plasma BNP level (ELISA) (A), and relative ANF and BNP (B and C) mRNA level in LV tissue, respectively, (real time RT-PCR). C, control vehicle group. n = 4–11 in each group. *, P < 0.05. (D) Semiquantitative RT-PCR of LV renin mRNA expression. Actin expression is shown below for loading control. (E) Quantification of renin mRNA expression normalized to actin mRNA expression. *, P < 0.05.
