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. Author manuscript; available in PMC: 2007 Nov 20.

Published in final edited form as: Endocrinology. 2001 Sep;142(9):4120–4130. doi: 10.1210/endo.142.9.8395

Fig. 3 — A. Empty CMV5 vector (CMV), CMV-hERα (hERα) or CMV-hERβ (hERβ) vectors were cotransfected in MDA-MB-231 cells with ERE2-TK-CAT reporter constructs and CMV-GAL internal reporter plasmid. Cells were grown for 36 h in the presence of control vehicle ethanol (C) or 10⁻⁸M E2. Results are expressed as the percentage of CAT activity in non infected cells (NI) and represent the mean ± SD (n = 5) of CAT activity after normalization for β-galactosidase activity. B. Non infected (NI) or Ad5, Ad-hERα, or Ad-hERβ infected MDA-MB-231 cells were transfected with ERE2-TK-CAT and CMV-GAL reporter constructs. Increasing MOI of Ad-hERα and Ad-hERβ viruses (0.1/1/10/100) were used. Cells were grown for 36 h in the presence of control vehicle ethanol (C) or 10⁻⁸M E2. Results are expressed as the percentage of CAT activity in non infected cells (NI) and represent the mean ± SD (n = 6) of CAT activity after normalization for β-galactosidase activity. C. MDA-MB-231 cells were infected with Ad-hERα and Ad-hERβ at MOI 100 and treated with increasing concentrations of E2. Results are expressed as the percentage of CAT activity in non infected cells (NI) and represent the mean ± SD (n = 6) of CAT activity after normalization for β-galactosidase activity. D. MDA-MB-231 cells were either transfected with empty CMV5 vector (CMV), CMV-hERα (hERα) or CMV-hERβ (hERβ) vectors or infected with Ad5, Ad-hERα or Ad-hERβ viruses along with ERE2-TK-CAT and CMV-GAL reporter constructs. Cells were grown for 36 h in the presence of control vehicle ethanol (C), 10⁻⁸M E2, ICI 164,384 (10⁻⁶M) or the combination of E2 and ICI 164, 384 (10⁻⁸ M and 10⁻⁶ M respectively). Results are expressed as the percentage of CAT activity in non infected cells (NI) and represent the mean ± SD (n = 6) of CAT activity after normalization for β-galactosidase activity.

Fig. 3 — A. Empty CMV5 vector (CMV), CMV-hERα (hERα) or CMV-hERβ (hERβ) vectors were cotransfected in MDA-MB-231 cells with ERE2-TK-CAT reporter constructs and CMV-GAL internal reporter plasmid. Cells were grown for 36 h in the presence of control vehicle ethanol (C) or 10⁻⁸M E2. Results are expressed as the percentage of CAT activity in non infected cells (NI) and represent the mean ± SD (n = 5) of CAT activity after normalization for β-galactosidase activity. B. Non infected (NI) or Ad5, Ad-hERα, or Ad-hERβ infected MDA-MB-231 cells were transfected with ERE2-TK-CAT and CMV-GAL reporter constructs. Increasing MOI of Ad-hERα and Ad-hERβ viruses (0.1/1/10/100) were used. Cells were grown for 36 h in the presence of control vehicle ethanol (C) or 10⁻⁸M E2. Results are expressed as the percentage of CAT activity in non infected cells (NI) and represent the mean ± SD (n = 6) of CAT activity after normalization for β-galactosidase activity. C. MDA-MB-231 cells were infected with Ad-hERα and Ad-hERβ at MOI 100 and treated with increasing concentrations of E2. Results are expressed as the percentage of CAT activity in non infected cells (NI) and represent the mean ± SD (n = 6) of CAT activity after normalization for β-galactosidase activity. D. MDA-MB-231 cells were either transfected with empty CMV5 vector (CMV), CMV-hERα (hERα) or CMV-hERβ (hERβ) vectors or infected with Ad5, Ad-hERα or Ad-hERβ viruses along with ERE2-TK-CAT and CMV-GAL reporter constructs. Cells were grown for 36 h in the presence of control vehicle ethanol (C), 10⁻⁸M E2, ICI 164,384 (10⁻⁶M) or the combination of E2 and ICI 164, 384 (10⁻⁸ M and 10⁻⁶ M respectively). Results are expressed as the percentage of CAT activity in non infected cells (NI) and represent the mean ± SD (n = 6) of CAT activity after normalization for β-galactosidase activity.