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. Author manuscript; available in PMC: 2007 Oct 24.
Published in final edited form as: J Neurochem. 2003 Dec;87(6):1427–1435. doi: 10.1046/j.1471-4159.2003.02113.x

Fig. 4.

Fig. 4

PAS antibody-immunoreactive proteins in mitochondria. SH-SY5Y cells were pre-incubated without and with wortmannin (40 nm, 30 min) prior to IGF-1 treatment (50 ng/mL, 15 min). (a) Purified cytosolic and mitochondrial proteins were separated in a 4–15% gradient SDS-polyacrylamide gel and immunoblotted with a phospho-Akt substrate (PAS) antibody. (b) Mitochondrial extracts from control, IGF-1, and wortmannin plus IGF-1 treated cells were immunoprecipitated with the PAS antibody and immunoblotted with ATP synthase β-subunit antibody and mitochondrial extracts were also immunoblotted for total levels of ATP synthase β-subunit.