Table 1.

Plasmids used

Plasmid	Comments	Ref.
pGem-7fZ(−)	Vector, resistant to ampicillin	Promega
pZMR100	Defective λ vector, resistant to kanamycin	30
pSP72	Vector, resistant to ampicillin	Promega
pT7-7	Expression vector, resistant to ampicillin	31
pKC796	ClaI-KpnI fragment (3.9 kbp) from φC31 encoding attP-int, resistant to apramycin	29
pKC1034	KpnI fragment (4 kbp) from S. ambofaciens encoding attB	S. Kuhstoss, Eli Lilly
pMS211	BamHI fragment (1.5 kbp) from φC31 repressor region in pUC19	37
pφC183	Sau3A-EcoRI fragment (0.5 kbp) encoding attP in an E. coli-Streptomyces shuttle plasmid	M. Brawner, SmithKline Beecham
pHS15	pZMR100 containing 2-kbp BsrI-TthIII fragment encoding φC31 int expressed from the tac promoter	This work
pHS21	KpnI-SalI attB fragment (0.5 kbp) from pKC1034 in pGEM7	This work
pHS23	attB fragment (0.5 kbp) in pSP72	This work
pHS20	BglII-EcoRI attP fragment (0.5 kbp) from pφC183 in pGEM7	This work
pHS22	attP fragment (0.5 kbp) in pSP72	This work
pHS282	attP fragment (84 bp) in pGEM7	This work
pHS33	attP fragment from pHS22 in pHS15	This work
pHS34	attB fragment from pHS23 in pHS15	This work
pHS44	attP and attB in pGEM7 in direct (POP′–BOB′) orientation	This work
pHS50	EcoRI fragment (0.4 kbp) encoding attL in pGEM7	This work
pHS52	AatII-SalI fragment (0.4 kbp) encoding attR in pGEM7	This work
pHS55	attL and attR in pGEM7 in orientation BOP′–POB′	This work
pHS61	pT7-7 containing int
pHS62	pT7-7 containing int for maximal expression	This work
pHS63	pHS63 encoding mutant S12F integrase	This work