Figure 1.

Sequence analysis of the evolved AspATs that have high activity for 2-oxovaline. (A) Lines show the entire coding region of the aspC gene. Longer bars indicate the nucleotide positions of missense mutations, which caused amino acid substitutions, and shorter bars indicate those of silent mutations. Asterisks are the missense mutations conserved in all five mutant AspATs. (B) Amino acid substitutions. Residues were numbered according to the sequence of cytosolic AspAT from pig as described (27).