Fig. 2.

Temporal patterns of Hsp23 and Hsp70 expression in S. crassipalpis show that Hsps are turned on at the onset of pupal diapause, continue to be expressed throughout diapause and the postdiapause period, and are turned off within hours after the pupae receive a signal to resume development. (A) Expression at the onset and early phase of pupal diapause (diapause begins 5d after puparium formation). (B) During late diapause and postdiapause development (postdiapause begins ≈60 d after puparium formation). Adapted from Hayward et al. (47). (C) Rapid decline in expression of Hsp23 and Hsp70 when pupal diapause is terminated artificially by the application of hexane. Adapted from Yocum et al. (17) and Rinehart et al. (18). ND, nondiapausing pupae; RE, red eye stage of pharate adult development. The 28s was used as the control gene.