Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Evaluation of degrons. Constructs 1 and 2, 3, or 4 were transfected into HeLa cells, and the ability of rapamycin to rescue luciferase activity was measured by Western blotting (construct 1, anti-MBP; constructs 2–4, anti-HA) (Upper) and luciferase activity (Lower). All luciferase activity was normalized to an internal renilla luciferase transfection control and performed in triplicate.
