Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Kinetic analysis of Smad3E transcriptional activation and rescue. HeLa cells were transfected with 1 and 7a and treated with rapamycin or a combination of rapamycin and SB43152. (A) Smad3E activity was determined by luciferase activity at different time points. All luciferase activity was normalized to an internal renilla luciferase transfection control and performed in triplicate. (B) Smad3E rescue was determined by Western blotting (anti-HA) at different time points.
