Fig. 6.

Chymotrypsin C stimulates trypsin-mediated trypsinogen activation (autoactivation) and trypsin-mediated trypsin degradation (autolysis). In the presence of millimolar Ca²⁺ concentrations, the trypsin degradation pathway is blocked and only the trypsinogen activation pathway is operational. Both pathways are affected by certain hereditary pancreatitis-associated mutations. Mutation A16V stimulates the activation pathway (20), whereas mutation R122H inhibits the degradation pathway. See text for details.