Table 3.

Effect of transformation on coding versus noncoding strand

ssOligo	WT	msh2	msh3	msh6	mlh1
COD(1)/NC(1) lead	28*	1.2	28*	1.2	1.2
DCOD(1)/DNC(1) lead	3.1*	2.1*	8.1*	0.3*	2.9*
COD(1)/NC(1) lag	8.8*	1.0	32*	3.8	1.1
DCOD(1)/DNC(1) lag	1.0	0.5*	2.5	0.1*	0.3*
Average	10	1.2	18	1.3	1.4
COD(7)/NC(7) lead	3.0*	0.6	0.6	0.3*	2.0
DCOD(7)/DNC(7) lead	1.4	2.6	2.0	1.6	3.0
COD(7)/NC(7) lag	0.4*	3.1*	4.6*	1.3	2.1
DCOD(7)/DNC(7) lag	0.5*	2.9*	3.5*	0.7	0.2
Average	1.3	2.3	2.7	1.0	1.8

The normalized transformation values shown in Fig. 2 were used to compute the indicated coding/noncoding ratios of ssOligo transformation. Note that the COD ssOligos will anneal to the noncoding, or transcribed, strand. The asterisk is defined in Table 1.