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. 2007 Jun 25;104(27):11370–11375. doi: 10.1073/pnas.0704964104

Fig. 6.

Fig. 6.

FAST does not require TIA1 or TIAL1 to activate to FGFR2 exon IIIb. (A) HEK293T cells were transfected with siRNAs targeting TIA1 or TIAL1 as well as the minigene pI12DE-WT and pcDNA3-Flag-FAST (1 μg). Proteins were extracted and monitored by Western blot analysis, and RNAs were analyzed for single and double-inclusion transcripts. (B) HEK293T cells were simultaneously transfected with TIA1 and TIAL1 siRNAs as well as pI12DE-WT and pcDNA3-Flag-FAST (1 μg) and analyzed as described for A.