Figure 3.

v-Myb inhibits RAR/RXR-DNA complex formation. EMSAs were performed with nuclear extracts from COS-7 cells either untreated (lanes 1–3) or transiently transfected with v-Myb expression vectors (pVM116) (lanes 4–6). A ³²P-labeled oligonucleotide containing the βRARE was used as a probe. The composition of DNA/protein complexes were determined by adding the following antibodies to the reaction mixtures: αRAR (lanes 2 and 5) and αMyb (lanes 3 and 6). In addition, two nonspecific complexes (NS) were observed.