Abstract
The fate of s.c. implants of fibrous, trypsin-purified human dermal collagen and collagen cross-linked with formaldehyde and glutaraldehyde has been studied in rats. Dermal collagen, and untreated skin implants, underwent resorption associated with a pronounced round-cell reaction. While collagen implants cross-linked with solutions of 0.04% and 0.08% formaldehyde became reduced in size, those cross-linked with 1% formaldehyde and 0.01%, 0.02% and 0.04% glutaraldehyde, although undergoing some collagen remodelling, retained their original size over the 25-week period of study. At 5 weeks the aldehyde cross-linked implants showed their greatest cellularity, reaching a lower, more stable cell population by 18 weeks. More round cells were seen at 5 weeks, particularly after formaldehyde cross-linking, than a later times when few were present. The results indicate that aldehyde-stabilized preparations of heterograft dermal collagen could have applications in the repair of tissue defects in man.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bowes J. H., Cater C. W. The interaction of aldehydes with collagen. Biochim Biophys Acta. 1968 Oct 21;168(2):341–352. doi: 10.1016/0005-2795(68)90156-6. [DOI] [PubMed] [Google Scholar]
- Cox R. W., Grant R. A., Kent C. M. An electron-microscope study of the reaction of collagen with some monoaldehydes and bifunctional aldehydes. J Cell Sci. 1973 May;12(3):933–949. doi: 10.1242/jcs.12.3.933. [DOI] [PubMed] [Google Scholar]
- Knapp T. R., Kaplan E. N., Daniels J. R. Injectable collagen for soft tissue augmentation. Plast Reconstr Surg. 1977 Sep;60(3):398–405. [PubMed] [Google Scholar]
- McGregor J. C., Lindop G. B. The behaviour of cialit-stored and freeze-dried human fascia lata in rats. Br J Plast Surg. 1974 Apr;27(2):155–164. doi: 10.1016/0007-1226(74)90008-3. [DOI] [PubMed] [Google Scholar]
- Oliver R. F., Grant R. A., Cox R. W., Hulme M. J., Mudie A. Histological studies of subcutaneous and intraperitoneal implants of trypsin-prepared dermal collagen allografts in the rat. Clin Orthop Relat Res. 1976 Mar-Apr;(115):291–302. [PubMed] [Google Scholar]
- Oliver R. F., Grant R. A., Hulme M. J., Mudie A. Incorporation of stored cell-free dermal collagen allografts into skin wounds: a short term study. Br J Plast Surg. 1977 Jan;30(1):88–95. doi: 10.1016/s0007-1226(77)90045-5. [DOI] [PubMed] [Google Scholar]
- Oliver R. F., Grant R. A., Kent C. M. The fate of cutaneously and subcutaneously implanted trypsin purified dermal collagen in the pig. Br J Exp Pathol. 1972 Oct;53(5):540–549. [PMC free article] [PubMed] [Google Scholar]
- Oliver R. F., Grant R. A. Reconstruction of full-thickness loss skin wounds using skin collagen allografts. Br J Plast Surg. 1979 Apr;32(2):87–90. doi: 10.1016/0007-1226(79)90002-x. [DOI] [PubMed] [Google Scholar]
- Rose A. G. Pathology of the formalin-treated heterograft porcine aortic valve in the mitral position. Thorax. 1972 Jul;27(4):401–409. doi: 10.1136/thx.27.4.401. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shakespeare P. G., Griffiths R. W. Dermal collagen implants in man. Lancet. 1980 Apr 12;1(8172):795–796. doi: 10.1016/s0140-6736(80)91294-5. [DOI] [PubMed] [Google Scholar]
- Woodroof E. A. Use of glutaraldehyde and formaldehyde to process tissue heart valves. J Bioeng. 1978 Apr;2(1-2):1–9. [PubMed] [Google Scholar]