Figure 6.

Western blot analyses of the effects of βAP on PKCα and PKCγ immunoreactivity after treatment with the protein synthesis inhibitor, cycloheximide (CHX), or phorbol ester (PMA). Visual inspection reveals that the decrease of PKCα immunoreactivity after exposure to 10 nM βAP(1–40) for 48 h was blocked by 30 min preincubation with 100 μM CHX (A). PKC activation with 100 nM PMA for 3 h restored the PKCα immunoreactive signal in βAP(1–40)-treated AC. PMA effect was blocked by preincubation with CHX. No modifications are visible for PKCα immunoreactivity in the AD group. (B) βAP(1–40)-mediated decrease of PKCγ immunoreactivity in AD cells was not affected because preincubation with either CHX or PMA was not able to reverse this effect.