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IFN-dependent tyrosine phosphorylation of STAT3 in empty vector or wild-type STAT3 transfected IFN-resistant Daudi cells. Proteins from control or IFN-treated (5,000 units/ml for 15 min) cell lysates were precipitated with anti-myc (Upper) or anti-STAT3 (Lower). The proteins were resolved by SDS/PAGE, blotted onto polyvinylidene difluoride membranes, probed with anti-pTyr or anti-STAT3, and visualized by enhanced chemiluminescence.
