Table 4. Summary of peptide/protein identifications using the AMT tag approach.
High Matched Confidence Unique |
All Unique |
CVa |
Peptide Dynamic Rangeb | ||||
---|---|---|---|---|---|---|---|
Peptide | Protein | Peptide | Protein | Mean | Median | ||
B3-CIT | 750 | 435 | 939 | 634 | 33.9 | 29.3 | 4585 |
B3-SERUM | 682 | 379 | 926 | 633 | 39.7 | 36.4 | 3524 |
B2-CIT | 792 | 455 | 1008 | 664 | 37.9 | 34.8 | 4361 |
B2-SERUM | 713 | 428 | 915 | 634 | 38.7 | 35.2 | 3717 |
B1-CIT | 864 | 441 | 975 | 572 | 19.0 | 13.5 | 3260 |
B1-SERUM | 847 | 425 | 937 | 542 | 19.9 | 15.4 | 4234 |
RefP | 860 | 447 | 1104 | 706 | 41.9 | 37.6 | 5028 |
RefS | 787 | 433 | 1016 | 701 | 41.7 | 37.6 | 5168 |
Coefficient of variation was calculated from average of the ion current values of all confident protein identifications.
Dynamic range was based on the highest and lowest observed ion current values of an identified peptide.