Table 2. Concentrations of DTDA that induces 50% inhibition in TcTIM and some of its mutants.

Enzyme	IC50 (µM)
TcTIM wild	0.258±0.035
TcTIM C118V	0.208±0.037
TbTIM V118C	NE
LmTIM	NE
TcTIM C15A	0.346±0.032
TcTIM C40A *	1.532±0.450
TcTIM C127A	0.057±0.0080

The effect of DTDA on the indicated enzymes was determined as in Figure 1, from the data the concentration that induced half-maximal inhibition was calculated. NE indicates that 200 µM DTDA had no effect.

^*As noted in the text, relatively low DTDA concentrations inhibit the activity by approximately 50%; significantly higher concentrations were required for full inhibition. The data in the Table indicate the concentration at which half-maximal inhibition of the initial phase was achieved.